A rapid screening assay for L452R and T478K spike mutations in SARS-CoV-2 Delta variant using high-resolution melting analysis

Rapid SARS-CoV-2 RT-LAMP assay setup using gene construct design approach

Background and Objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still an ongoing challenge in health system worldwide. Molecular detection methods including reverse transcription loop-mediated isothermal amplification (RT-LAMP) can help cutting off the transmission chain of the virus. In this study, prompt setting up of the RT-LAMP assays were investigated using ge...

Analysis of Sars-CoV-2 RBD Mutations in Khuzestan Province, Iran—A Retrospective Study, 2021

Background and Objective: One of the main reasons for the ongoing pandemic is the substitutions in the Receptor binding domain(RBD), which is involved in binding to the ACE2 receptor. Methods: Oro-nasopharyngeal swabs were collected from 41 confirmed patients between September 2020 and May 2021 to analyze the mutations of SARS-CoV-2 RBD in Khuzestan Province. Bi-Directional sequencing was use...

Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene

Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic ...

Analysis of HIV diversity using a high-resolution melting assay.

HIV viruses are usually genetically homogeneous shortly after infection, and become more heterogeneous over time. We developed a high-resolution melting (HRM) assay to analyze HIV diversity without sequencing. Plasma samples from the HIVNET 012 trial were obtained from nine Ugandan mother-infant pairs. DNA amplified from the HIV gag region was analyzed to determine the number of degrees over wh...

rapid quantitative determination of gstp1 promoter methylation level using differential high resolution melting analysis (d-hrma)